Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells

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09/18/2019

Benyamin Shahbazi, Zahra Salehi Najafabadi, Hamidreza Goudarzi, Mahnaz Sajadi, Fatemeh Tahoori, Masoumeh Bagheri

Journal of Venomous Animals and Toxins including Tropical Diseases 2019;25:jvatitd-2019-0009
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100319&lng=en&nrm=iso&tlng=en | © The Author(s). 2019
Received: February 27, 2019 | Accepted: August 06, 2019 | Published: September 16, 2019

ABSTRACT

Background

Several studies have pointed out that certain snake venoms contain compounds presenting cytotoxic activities that selectively interfere with cancer cell metabolism. In this study, Pseudocerastes persicus venom and its fractions were investigated for their anticancer potential on lung cancer cells.

Methods

Lung cancer cells (A549) and normal fibroblast cells (Hu02) were treated with the P. persicus venom and its HPLC fractions and the cell cytotoxic effects were analyzed using MTT and lactate dehydrogenase release assays. Apoptosis was determined in venom-treated cell cultures using caspase-3 and caspase-9 assay kits.

Results

The treatment of cells with HPLC fraction 21 (25-35 kDa) of P. persicus venom resulted in high LDH release in normal fibroblast cells and high caspase-3 and caspase-9 activities in lung cancer cells. These results indicate that fraction 21 induces apoptosis in cancer cells, whereas necrosis is predominantly caused by cell death in the normal cells. Fraction 21 at the final concentration of 10 μg/mL killed approximately 60% of lung cancer cells, while in normal fibroblast cells very low cell cytotoxic effect was observed.

Conclusions

HPLC fraction 21 at low concentrations displayed promising anticancer properties with apoptosis induction in the lung cancer cells. This fraction may, therefore, be considered a promising candidate for further studies.

PubMed Central https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748451/

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KEYWORDS

Pseudocerastes persicus venom
Persian horned viper
HPLC fractions
Cytotoxicit]
Apoptosis
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