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Nitro-heterocyclic compounds induce apoptosis-like effects in Leishmania (L). amazonensis promastigotes

Daiane Barros Dias Mendonça, Renata Ellen Costa Silva, Fanny Palace-Berl, Cleusa F. H. Takakura, Sandra Regina C. Soares, Lucia Maria Almeida Braz, Leoberto Costa Tavares and Jose Angelo Lauletta Lindoso

J Venom Anim Toxins incl Trop Dis, 2019, 25:e144418
Received: 03 May 2018 | Accepted: 30 November 2018 | Published online: 11 March 2019
Collection: Novel hit and lead compounds against neglected protozoan diseases
https://doi.org/10.1590/1678-9199-JVATITD-1444-18

Abstract

Background: Three drugs - pentavalent antimonials, amphotericin B and pentamidine - are currently used for leishmaniasis treatment. They are administered for long periods, only parenterally, and have high cardiac, renal and hepatic toxicities. Therefore, the investigation of new compounds is required. Nitro-heterocyclic derivatives have been used as possible drug candidates to treat diseases caused by trypanosomatids. Methods: Leishmania (L.) amazonensis promastigotes (MHO/BR/73/M2269), maintained in the Laboratório de Soroepidemiologia - Instituto de Medicina Tropical- USP, were exposed to five nitroheterocyclic derivatives, with differences at phenyl-ring position 4: BSF-C4H9, BSF-H, BSF-NO2, BSF-CH3 and BSF-Cl, for 48 hours. After analyzing viability (MTT assay), we evaluated cellular-morphology activity of compounds by transmission electron microscopy (TEM) and measurement of apoptosis (phosphatidylserine expression) by flow cytometry. Results: EC50 of amphotericin B and BSF-CH3 were 0.50 µM and 0.39 µM respective. Other nitro-heterocyclic compounds presented EC50 higher than amphotericin B. All compounds showed greater AV- and PI-positive expression than amphotericin B at 100 µM, except BSF-NO2. TEM showed complete nuclear disfigurement with 100 µM of BSF-NO2, 25 and 6.25 µM of BSF-H, and 6.25 µM BSF-Cl; presence of vesicles within the flagellar pocket with 25 µM BSF-H; alteration of the kinetoplast with 25 µM BSF-C4H9, 25 µM of BSF-H, 6.25 µM BSF-CH3 and 6.25 µM of BSF-Cl. Conclusions: Nitro-heterocyclic compounds have shown activity against promastigotes of L. amazonensis, at lower concentrations. However, improvement of compound scaffolds are needed to assist the elucidation of the mechanism of action and to achieve greater activity. 

 

Keywords: Leishmaniasis treatment, Nitro-heterocyclic compounds, Leishmania (L.) amazonensis.

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